The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

Abstract The purpose of the present study was to evaluate de influence protein–sugar complexation on stability and functionality C-reactive protein, after exposure constant high temperatures, in order develop highly stable positive controls for in-vitro diagnostic tests. protein is a plasmatic used as biomarker diagnosis series health problems such ulcerative colitis, cardiovascular diseases, metabolic syndrome, due its essential role evolution chronic inflammation. sugar–protein interaction investigated using steady state time resolved fluorescence. results revealed that there are more than two classes tryptophan, with different degree accessibility quencher molecule. Our also complexes have superior thermostability, especially gamma irradiation at 2 kGy, being functional even 22 days 40 °C.